© 2017 by the Society of Nuclear Medicine and Molecular Imaging. These data support the use of CLI for the evaluation of radiotracer performance.Ĭerenkov luminescence imaging (CLI) ImmunoPET antibody. Moreover, CLI provided clear discrimination between uptake kinetics of an intact IgG and its small-molecular-weight derivative Mb.
Targeting data generated by CLI correlated with that generated by PET and necropsy.ĬLI provided a rapid and simple assessment of the targeting specificity and pharmacokinetics of the antibody-based PET radiotracers that correlated well with the behavior observed by standard PET imaging.
Under the experimental conditions used, PSMA-selective uptake of 64Cu-NODAGA-PSMA-IgG and 64Cu-NODAGA-PSMA-Mb was observed by CLI as early as 3 h after injection, with tumor-to-background ratios peaking at 24 (IgG) and 16 (Mb) h after injection. If the item you received doesnt match the listing description, your. Tumor targeting was evaluated by both PET and CLI.Ħ4Cu-NODAGA-PSMA-IgG and 64Cu-NODAGA-PSMA-Mb retained the ability to bind cell surface PSMA, and both radiotracers exhibited selective uptake into PSMA-positive tumors. From Inglewood, California, United States. 64th Pl, Inglewood, CA - Owners, Taxes, Deeds, Records, Sales, Permits. Immunoreactive preparations of the radiolabeled antibodies were injected into NCr nu/nu mice harboring PSMA-positive CWR22Rv1 and PSMA-negative PC-3 tumor xenografts. The anti-prostate-specific membrane antigen (PSMA) huJ591 antibody (IgG 150 kDa) and its minibody (Mb 80 kDa) format were functionalized with the chelator 1,4,7-triazacyclononane-1-glutaric acid-4,7-diacetic acid (NODAGA) and radiolabeled with the positron-emitting radionuclide 64Cu (half-life, 12.7 h). In this study, we investigated the use of Cerenkov luminescence imaging (CLI) as compared with PET as a modality for evaluating the in vivo behavior of antibody-based radiotracers.
The development of antibody-based radiotracers requires extensive characterization of their in vitro and in vivo properties, including their ability to target tumors in an antigen-selective manner. She enjoys helping people heal and getting them back to doing the activities they love, as well as educating her patients on their condition, treatment, and rehabilitation.Įrica is an active person, with a past history of triathlons and marathons. She also enjoys pilates and yoga, and is very active with her 4 children, taking them biking, rollerblading, and skateboarding.Antibodies, and engineered antibody fragments, labeled with radioisotopes are being developed as radiotracers for the detection and phenotyping of diseases such as cancer. The majority of her experience has been in an outpatient Orthopedic setting, ranging from teens to adults, athletes, post surgical patients, motor vehicle accident patients, sports injuries, and rehabilitation. She has been working in her field for over 20 years. She regularly participates in continuing education classes to stay up-to-date on new techniques and other tools that can help her treat patients to the best of her ability. Physical Therapist Erica Fleischauer received both her Bachelor of Science Biology and Master of Science Exercise Physiology at Florida State University. She received her Master of Physical Therapy at University of St. She’s also an advocate for Breast Cancer awareness and is in the process of starting a Non-Profit to help educate and give support to people and their families going through breast cancer.
In addition to running, she enjoys the outdoors, CrossFit, Krav Maga, athletic training, and working with kids. Leslie took up running in 2016 with the goal to accomplish 40 races in one year, and she nearly met that goal with such races such as the 39 Mile Avon Breast Cancer Walk in Washington, D.C., Los Angeles Marathon, Bolder Boulder 10k, multiple Spartan Races, Mount Charleston Marathon, etc. Prior to becoming a team member at Goodlife Physical Medicine, she spent several years working at different integrated pain management clinics which specialized in non-surgical spinal decompression, chiropractic, physical therapy, and pilates. Understanding the Bible With Pastor Lee 5:30pm - 6:10pm, Sweet Heaven with PYO,In-Bong and YUN, Yu-Seon 6:10pm - 6:50pmreligion, Pastor HA, Yong-Jos John 6. Leslie is a lifelong learner and has taken numerous courses since graduation. in Health Education from California State University Northridge in 2000, and then received her Master of Science in Physical Therapy degree from California State University Northridge in 2003.
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